Composite

Part:BBa_K4607006:Design

Designed by: Axel Rojero   Group: iGEM23_Tec-Chihuahua   (2023-07-21)


Expression cassette for LysCSA13-ABD protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1100
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1100
    Illegal SpeI site found at 127
    Illegal NotI site found at 1242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1100
    Illegal BglII site found at 30
    Illegal BamHI site found at 1094
    Illegal XhoI site found at 1251
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1100
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1100
    Illegal XbaI site found at 96
    Illegal SpeI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for expression in Escherichia coli. It also contains flexible linkers to the optimum domain activity.


Source

Staphylococcus phage CSA13 (GenBank: MH107118.1:5844-6593), Affinity-matured variant Streptococcus strain G148 protein G (Jonsson et al., 2008)

References